Are the tests working against a factor to be calibrated?

Yes, all the tests are to be calibrate4d before use. The kinetic tests, like ast.., can work against a calibrator ( in this case the analyzer will calculate an "experimental" factor, based on the effective reaction's o.d.) or against a "theoretical" factor. In this case you have to put in the "cal value" box "0" and in the "factor value" the number indicated in the applicative method, based on different parameters like total volume, sample volume, optical path and millimolar extinction coefficient. The analyzer will perform only a "blank" update and will utilize the imposed factor.